Cloning, Expression, Purification, and Characterization of Clostridium botulinum Neurotoxin Serotype F Domains
The use of recombinant BoNT domains has been proposed as a means to develop strategies to treat and prevent botulism.Here, details on the molecular cloning, protein expression, purification, and immunoreactivity of BoNT/F domains from Clostridium botulinum are presented.Initially, full-length synthetic genes encoding recombinant BoNT/F domains (cat